摘要
目的研究针对胰岛素基因增强结合蛋白1(ISL1)的小干扰RNA(siRNA)对人胃癌干细胞特性的影响。方法体外合成ISL1的siRNA,转染人胃癌细胞系BGC823和BGC-S,逆转录酶链式反应(RT-PCR)和免疫印迹法检测ISL1基因和蛋白表达的变化,MTT比色法测定细胞存活率。结果构建的针对ISL1基因的siRNA能够显著抑制靶基因ISL1 mRNA和ISL1蛋白的表达。转染48h后BGC823和BGC-S细胞的存活率明显下降。结论 siRNA能明显抑制胃癌细胞BGC823和BGC-S的ISL1表达,从而对进一步研究胃癌干细胞奠定了基础。
Objective To research the effect of inhibition of small interference RNA（siRNA） insulin gene enhance binding protein 1 （ ISL1 ） expression on gastric cancer stem cell characteristics. Methods Synthetized siRNA of ISL1 in vitro. And the human gastric cancer cell lines BGC823 and BGC-S were transfected. The ISL1 gene and its protein expression changes were detected by reverse transcriptase polymerase chain reaction （RT-PCR） and western blotting. The cell survival rate was measured by MTY assay. Results The siRNA targeting the ISL1 gene can inhibit the expression of target gene ISL1 mRNA obviously, and can inhibit ISL1 protein expression. After 48 hours, the survival rate of BGC823 and BGC-S cell declined dramatically. Conclusion siRNA could significantly inhibit the gastric cancer cell line BGC823 and BGC-S ISL1 expression, in order to lay the foundation for further study of gastric cancer stem cells.
出处
《临床合理用药杂志》
2013年第5期3-5,共3页
Chinese Journal of Clinical Rational Drug Use
