GM-1治疗脊髓损伤的实验研究

Experimental Study on Treatment of Acute Spinal Cord Injury with GM-1 in Rats

为评价单唾液酸四己糖神经节苷脂（GM－1）对急性脊髓损伤（ASCI）的治疗效果，选取55只SD大鼠随机分成对照组、损伤组和治疗组，用Allen氏法致伤大鼠T3～L1脊髓节段。治疗组伤后15、90、180min，第2个及第3个24h分别腹腔内注射GM－110mg／kg，损伤组伤后注射生理盐水。伤后1、6及24h，1及6周时各取损伤组和治疗组鼠2只的伤区脊髓组织送光镜和电镜检查，并行Rivlin斜板试验评价大鼠运动功能。结果：治疗组脊髓灰质出血坏死减轻，周围白质大部存留，髓鞘残留，大量毛细血管和少突胶质细胞增生，并且运动功能逐渐恢复。提示：GM－1对伤段脊髓具有保护作用，并且可以促进ASCI大鼠的运动功能恢复。

The therapeutic effects of monosialutetrahexosyl ganglioside (GM-1) in rats with acute spinal cord injury (ASCI) were assessed. Fifty-five rats were randomly divided into control group and injured group A and injured group B. Allen method was used to injury the segments of T3-L1 of the rats. In injured group A, GM-1 (10 mg/kg)was injected intraperitoneally at 15 min. 90 min, 180 min, the second day and third day after trauma. Injured group B and control group were subjected to the injection of normal saline. One rat in each time point of 1 h, 6 h, 24 h, one week and six weeks after trauma in injured groups A and B underwent light and electron microscopic examination of the injured spinal cord tissue. Revlin test was per formed to evaluate motor function of the rats. The results showed that in injured group A the hemorrhage and necrosis of the grey matter were a1leviated, the surrounding white matter survived, capillaries and oligodentrocytes abundantly proliferated, and motor function gradually recovered. It was concluded that GM-1 could improve the spinal cord microcirculation, decrease the secondary patho1ogical damage markedly. and promote the recovery of motor function in the rats with ASCI.