摘要
了解转化生长因子 - β(TGF- β)诱导骺板软骨细胞增殖的作用。方法 :采用鸡胚股骨无血清培养、PCNA(细胞核增殖抗原)免疫染色方法研究对骺板软骨细胞增殖的作用。结果 :PCNA阳性软骨细胞数目与分布部位与TGF - β 作用剂量、时间密切相关,PCNA阳性细胞分布区域和数目远多于对照组,根据染色情况可以将PCNA阳性细胞分为S期和非S期细胞二种。结论 :TGF- β 能诱导非增殖软骨细胞表达PCNA,用PCNA作为细胞增殖的指标是不适宜的。
Objective:To understand the effect of transforming growth factor-beta on epiphtseal plate chondrocyte proliferation.Methcod:We detected PCNA expression of chondrocyte of embryonic chick femora which were cultured in serum-free and treated with TGF-β.Results:The results showed the number and distribution of positive chondrocyte of PCNA had close relations with effect of TGF-β in varied time and dosage,We also found that PCNA positive chondrocytes could divide into two types,s -phase and non s-phase according staining condition.Conclusion:These data suggest TGF-β cound induce expression of PCNA in nonproliferation chondrocytes and the PCNA immunohistochemical staining were not suitable for detection of proliferation.
出处
《中国口腔种植学杂志》
2000年第2期51-53,F002,共4页
Chinese Journal of Oral Implantology