原位杂交检测子宫内膜癌中金黄色葡萄球菌L型DNA

DETECTING OF STAPHYLOCOCCUS AUREUS L-FORM DNA IN ENDOMETRIAL ADENOCARCINOMA WITH IN SITU HYBRIDIZATION TECHNIQUE

目的 研究细菌L型感染与子宫内膜腺癌的相关性。方法 用革兰染色、免疫组化和DNA核酸探针原位杂交等方法 ,对 6 6例子宫内膜腺癌 ,30例子宫内膜增生症进行检测。结果 子宫内膜腺癌和内膜增生症中革兰氏染色细菌L型阳性率分别为 5 4 5 % ,5 6 7% ;免疫组化染色 (S -P法 )检测阳性率分别为 5 6 1%和 6 0 6 % ;2 0例子宫内膜腺癌标本 ,用金黄色葡萄球菌 (金葡菌 )L型地高辛标记的核酸探针作原位杂交 ,结果发现 45 %的宫内膜腺上皮细胞核内显示有金葡菌L型DNA阳性颗粒。结论 提示细菌及其L型可侵入子宫内膜腺上皮细胞 ,导致细胞异常增生甚至肿瘤发生。证明子宫内膜腺癌的发生与细菌L型感染有一定的相关性。

Aim To study the relativity between the infection of bacterial L-forms and endometrial adenocarcinoma Methods 66 cases of endometrial adenocarcinoma and 30 cases of endometrial hyperplasia were detected with Grams stain,immunohistochemical and in situ hybridization techniques Results With the Grams staining the positive rate of bacterial L-forms in endometrial adenocarcinoma and endometrial hyperplasia were 54 5% and 56 7% In immunohistochemical staining with S-P method, S aureus L-form antigen was positive in 56 1% and 60 6% 20 cases of endometrial adenocarcinoma were examined with degoxin labelled S aureus DNA probe,positive granules of S aureus DNA in blue color were found distributing evenly inside the nuclei in 45% of the cases Conclusion The S aureus L-form DNA may invade into the glandular epithelial cells of endometrium and lead to hyperplasia and cancer formation This indicates the existence of a relationship between the infection of S aureus and its L-forms and the development of cancer in endometrium