牛血清白蛋白稳定水溶性纳米锗的制备、表征及其形成机理

Synthesis and Characterization of BSA Stabilized Germanium Nanoparticles in Aqueous Solution and Its Formation Mechanism

在水溶液中利用牛血清白蛋白(BSA)作稳定剂制备水溶性良好的纳米锗颗粒,提供了一种合成蛋白质修饰的纳米锗材料的新思路。采用透射电子显微镜(TEM),粒度分析及能谱分析(EDS),对制备的纳米锗的形貌和组成进行表征,探讨各实验条件变化对纳米锗形貌的影响,进一步分析纳米锗的形成机理。结果表明球形纳米锗颗粒平均粒径约为60nm,分散性良好,调控诱导出了方形和棒状的纳米锗材料。本研究方法简单、可控且重复性好,BSA做稳定剂大大提高了纳米锗在水溶液中的稳定性,同时改善了纳米锗材料的生物相容性,为拓展纳米锗材料在生物领域的应用研究提供基础。

Germanium （Ge） nanoparticles of 60 nm were synthesized in aqueous solution by using bovine serum albumin （BSA） as a stabilizer, which provided a new method to prepare good dispersion of water-soluble Ge nanoparticles. Transmission electron microscopy （TEM）, granulometer and energy dispersive spectroscopy （EDS） were used to characterize the morphology and chemical composition of Ge nanoparticles. A possible mechanism was proposed to investigate the formation process of Ge nanoparticles. Results showed that square- liked or rod-liked Ge nanoparticles were synthesized through the regulation of different experimental conditions. BSA-based stabilizer can effectively improve the dispersion and biocompatibility of Ge nanoparticles, which provides a new way to stabilize Ge nanoparticles, and further applications in biomedicine.