蛋白片段互补分析方法及其应用

Protein Fragment Complementation Assay(PCA)and the Applications

蛋白片段互补分析技术是近年发展起来的一种分析蛋白质-蛋白质相互作用的新方法。典型的蛋白片段互补分析技术已经成功地利用二氢叶酸还原酶,β-内酰胺酶,绿色荧光蛋白以及荧光素酶片段互补进行胞内蛋白分子相互作用研究。这一技术不仅可以动态、定位分析细胞内蛋白质分子相互作用、绘制细胞内信号传导、蛋白质生物化学网络,还可以应用到蛋白质文库、cDNA文库和高通量药物筛选等。综述了蛋白片段互补分析技术的原理、方法及其应用。

Protein fragment complementation analysis （PCAs） is a recently developed new technique to assay protein-protein interactions. Typical PCA has been using dihydrofolate reduetase, β-laetamase, GFP and luciferases to study protein-protein interactions successfully. This technology can not only analysis the localization of protein molecular interaction dynamically, draw intracellular signal transduction, protein biological chemical network, but also can be applied to protein library and high throughput new drug screening. In this review, we summarized the concept, methodology and the applications of PCA.