苹果锈果类病毒属属级寡核苷酸芯片筛查方法的建立

Establishment of the Screening Oligonucleotide Microarray of Apscaviroid in Genus Level

苹果锈果类病毒属是重要的植物类病毒属,目前尚无有效的筛查方法。通过对该属类病毒的核苷酸序列进行分析筛选,设计了35条用于该属类病毒筛查的属级特异性探针并制备了寡核苷酸芯片。应用苹果锈果类病毒及柑橘矮化类病毒标准样品对该芯片进行验证,结果表明所建立的属级芯片可以获得有效的杂交信号,其灵敏度与RT-PCR电泳法相当。该芯片可用于苹果锈果类病毒属类病毒的筛查,为该属类病毒的检疫与防控提供技术支撑。

Apscaviroid is a significant plant viroid genus and has not screening method available at present. For the establishment of the screening oligonueleotide microarray of Apscaviroid in genus level, thirty-five genns specific probes from the sequences ofApscavlroid viroid were designed. Microarray was constructed based on these probes and identificated with Apple scar skin viroid and Citrus dwarfing viroid standard samples. Results showed a high specificity for detection of Apscaviroid viroid and the same detection sensitivity with RT-PCR. The microarray can be used to screen and provide technical support of quarantine and control for Apscaviroid viroid.