胶质源性神经营养因子对脊髓损伤后前角运动神经元的保护作用

Protective effect of glial cell line-derived neurotrophic factor on spinal front corner motor neurons

目的 :探讨胶质细胞源性神经营养因子 (GDNF)对脊髓损伤后运动神经元的保护作用。 方法 :大鼠分为假手术组、生理盐水 (NS)组和 GDNF组 ,改良 Allen法撞击致伤 T1 2 脊髓 ,蛛网膜下腔分别给予 NS和 GDNF,分别取不同时间的伤段脊髓进行切片 ,应用酶组织化学染色方法显示脊髓前角外侧核运动神经元中胆碱酯酶 (Ch E)和酸性磷酸酶 (ACP)活性 ,并结合计算机进行图像分析。结果 :GDNF组较 NS组 Ch E活性显著增加 ,ACP活性显著降低 ;2 1d时 NS组的 ACP及 Ch E活性均高于及低于假手术组 ;GDNF组与假手术组 Ch E和 ACP活性差别不显著。 结论

Objective:To investigate the protective effect of glial cell line derived neurotrophic factor (GDNF) on spinal front corner motor neurons. Methods:Animals were divided into 3 groups: false operation group, NS and GDNF group. NS and GDNF were given through subarachnoid cavity after T 12 segments of spinal cord were injured by modified Allen crush method. The specimen of spinal cord were taken at different time and sectioned. Enzyme histochemical technique of acid phosphatase (ACP) and cholinesterase (ChE) was conducted. Image analysis of section was done with computer. Results:(1)The activity of ACP in GDNF group was obviously lower than that in NS group, and ChE activity in GDNF group was notablely higher than that in NS group. (2) The activity in ACP or ChE in NS group was higher or lower than that in false operation group at 21 days. There was no marked difference of ACP and ChE activities between false operation group and GDNF group. Application of GDNF significantly improved the above mentioned enzymatic activity changes. Conclusion:GDNF plays a role in protecting the spinal motor neurons against injury.