牙周炎牙龈中表皮生长因子受体表达的研究

The expression of receptor of epidermal growth factor in gingiva of patient with periodontitis

目的：观察表皮生长因子受体（ＥＧＦＲ）在牙周炎中的表达。方法：采用免疫组化方法，检测１５例健康人、３２例成人牙周炎（ＡＰ）和１２例青少年牙周炎（ＪＰ）患者牙龈的ＥＧＦＲ表达。结果：正常牙龈ＥＧＦＲ仅局限于基底细胞膜；在ＡＰ患者牙周袋内壁或结合上皮，大部分上皮细胞膜存在ＥＧＦＲ高水平表达，基底层最强烈，随角化细胞的分化而逐渐减弱消失；ＪＰ患者牙龈亦出现局限于上皮生发展的高水平表达。ＡＰ与ＪＰ之间或与正常牙龈之间差异非常显著（Ｐ＜０．０１）。结论：ＥＧＦＲ可能影响着结合上皮向根方迁移的过程，在ＡＰ和ＪＰ的发生发展中起着一定的作用。

AIM:To observe the expression of epidermal growth factor receptors(EGFR) of gingival tissue in periodontitis. METHODS: The expression of EGFR was determined by using immunohistochemical techniques in gingival tissue of 15 healthy individual, 32 cases with adult periodontitis (AP) and 12 cases with juvenile periodontitis (JP). RESULTS: Expression of EGFR was mainly located on basal cell membranes in healthy gingiva, and the staining intensity was faint. In AP cases, expression of high level EGFR was mostly observed on the membranes of epithelial cell in the periodontal endopocket or junctional epithelium, intensity of staining appeared to decrease gradually with the differentiation of keratinocytes, and the horny cell layer was not stained by the antibody. In JP cases, strong positive staining was present on membrane of epithelial cells in the germinative layer of gingival tissue. There was an apparent difference between healthy gingiva and AP or JP (P＜ 0.01 ), or AP and JP (P＜ 0.01). CONCLUSION: The distribution and expression of EGFR in gingival epithelium of periodontitis showed obvious differ- euce. The data indicated that EGFR may affect apical migration of junctional epithelium, and may play a role in development of AP and JP.