分子生态学在生物膜微生物研究中的应用前景

Prospects of molecular ecology in biofilm microbiology research

利用分子生态学技术分析研究生物膜内细菌种群,对有效处理污水,提高氮磷去除率有着极为重要的意义。本文介绍了提取总DNA、PCR、DGGE、RFLP、SSCP和FISH等常见分子生态学技术,综述了国内外的研究进展,探讨了分子生态学技术在生物膜内微生物群落研究的应用前景,为探寻生物膜内微生物的多样性、结构与分布以及动态性拓宽了思路。

Use molecular ecology technology to analyze bacteria populations in biofilm, which has a very important significance for effective sewage treatment and improvement of nitrogen and phosphorus removal rate. This article described the extraction of total DNA, PCR, DGGE, RFLP, SSCP, FISH and other common molecular ecology techniques, summarized researches at domestic and foreign, discussed the application prospects of molecular ecology techniques for study of microbial communities in biofilm, broadened the idea of exploring the diversity of microorganisms, the structure and distribution, as well as the dynamic.