喉鳞癌微血管密度与颈淋巴结转移及病理临床分期的相关性研究

Correlation of intratumoral microvessel density with metastasis and clinicopathologic stages in squamous cell carcinoma of the larynx

目的 研究喉鳞癌微血管密度与颈淋巴结转移及病理临床分期的相关性。方法 采用抗Ⅷ因子相关抗原的抗体标记肿瘤血管内皮细胞并测定喉鳞癌组织微血管密度。结果 ①颈淋巴结转移组喉鳞癌的微血管密度显著高于非转移组 (32 .1518± 6 .4 89∶18.0 6 72± 4 .983,P <0 .0 1) ;②喉鳞癌微血管密度大于均数组则颈淋巴结转移率显著增高 (P <0 .0 1) ;③病理临床分期晚期组喉鳞癌的微血管密度明显高于早期组 (P <0 .0 1)。

Objective To investigate correlation between tumor angiogenesis and cervical lymph node metastases in laryngeal carcinoma. Methods Microvessels were stained by immunohistochemistry based on monoclonal antibody against factor Ⅷ related endothelial antigen and intratumoral microvessel density was measured. Results ①Laryngeal carcinomas with cervical lymph node metastasis had a significantly higher microvessel density than those without metastasis ( 32.1518 ± 6.489 , 18.0672 ± 4.983 , P< 0.01 ); ②Laryngeal carcinomas with microvessel density≥23 had a significantly higher risk of metastasis than those with microvessel density<23 (P< 0.01 ); ③Laryngeal carcinomas at clinical stagel Ⅱ had a significantly lower microvessel density than those at clinical stage Ⅲ-Ⅳ. Conclusion Microvesscl density may be a good index in predicting cervical lymph node metastasis of laryngeal carcinoma.