p16和bcl-2蛋白在眼睑恶性肿瘤中的表达及其意义

A study on the expression of p16 protein and bcl-2 protein in cutaneous eyelid tumors

目的 探讨肿瘤抑制基因p16和细胞凋亡抑制基因bcl 2与眼睑恶性肿瘤发生、发展的关系。方法 应用链霉亲和素 生物素 过氧化物酶复合物免疫组化法检测 96例常见眼睑恶性肿瘤组织中p16和bcl 2蛋白的表达。结果 在 96例眼睑恶性肿瘤组织中 ,p16蛋白阳性表达均为细胞核染色 ,其阳性表达率分别为基底细胞癌 (basalcellcarcinoma,BCC) 70 0 % (2 8/ 40 ) ,鳞状细胞癌 (squamouscellcarcinoma,SCC) 5 4 6 % (18/ 33) ,眼睑皮脂腺癌 5 6 5 % (13/ 2 3) ;p16蛋白的阳性表达与肿瘤分化程度呈负相关 ,高分化癌与低分化癌间比较差异有显著性 (χ2 =5 19,P <0 0 5 )。bcl 2蛋白的阳性表达均为细胞浆染色 ,40例BCC中 ,bcl 2蛋白的阳性表达率为 10 0 0 % ;33例SCC中 ,仅有 1例呈局灶性阳性反应 ,32例染色较弱 ;2 3例眼睑皮脂腺癌者均未见bcl 2蛋白的阳性表达。结论 p16蛋白的表达与眼睑恶性肿瘤的发生及分化程度有关。bcl 2蛋白在BCC中为普遍过度表达 。

Objective To investigate the relationship between p16 (tumor suppressor) and bcl 2 (apoptosis inhibitor) gene expression and the incidence and development of eyelid malignant tumors Methods Streptavidin biotin peroxidase complex immunohistochemical method was used to study the expression of p16 gene and bcl 2 gene in 96 cases with eyelid malignant tumors Results In the 96 cases, including 40 cases of basal cell carcinoma (BCC), 33 squamous and 23 sebaceous carcinomas, their p16 protein positive (nuclear staining) rates were 70 0%, 54 6% and 56 5% respectively The p16 positive rate was negatively correlated to the degree of tumor histological differentiation, and the rate difference between the high differentiated carcinoma and the low differentiated one was significant ( P <0 05) bcl 2 protein positive expression was detected in the cytoplasm All of the 40 BCCs were bcl 2 positive and nearly all the tumor cells showed positive cytoplasmic expression, while in the 33 specimens of squamous cell carcinoma, only did one show positive focal reaction and the staining in other 32 cases was relatively faint None of the 23 sebaceous carcinoma expressed bcl 2 Conclusions The expression of p16 protein is related to the occurrence and degree of differentiation of eyelid malignant tumors The over expression of bcl 2 protein suggests that suppression of apoptosis might play a role in the tumorigenesis of BCC