牛磺酸抑制过氧化氢诱发牛晶状体上皮细胞凋亡的实验研究

An experimental research of taurine on H_2O_2-induced bovine lens epithelial cell apoptosis

目的 观察牛磺酸对过氧化氢 (H2 O2 )诱发牛晶状体上皮细胞凋亡的抑制作用。方法采用牛晶状体器官培养及DNA缺口末端原位标记法检测牛磺酸对H2 O2 诱发的牛晶状体上皮凋亡细胞数量的影响 ,并进行相关比较及统计学分析。结果 晶状体上皮细胞凋亡发生于晶状体混浊之前 ;牛磺酸可明显抑制H2 O2 所致的牛晶状体上皮细胞凋亡。结论 牛磺酸可抑制因氧化损伤诱发的牛晶状体上皮细胞凋亡 。

Objective To study the inhibitory role of taurine on H 2O 2 induced bovine lens epithelial cell apoptosis Methods By terminal deoxyribonucleotide transferase mediated dUTP nick end labeling (TUNEL) detection and bovine lens organ culture, we examined the affect of taurine on the number of H 2O 2 induced bovine lens epithelial cells with apoptosis, the related comparisons and statistic analyses were carried out Results (1) Lens epithelial cell apoptosis began before lens opaqueness (lens became opaque after 6 hours of incubation, while the lens epithelial cell apoptosis was detected after 3 hours of incubation); (2) Taurine could apparently inhibit H 2O 2 induced bovine lens epithelial cell apoptosis Conclusion Taurine has an inhibitory role on H 2O 2 induced bovine lens epithelial cell apoptosis and can delay and ameliorate the occurrence and development of cataract