宫颈癌组织中HPV16.18E6蛋白的表达及其与p53蛋白表达的关系

Expression of HPV16 and 18E6 protein in Cervical Carcinomas and its Correlation with p53 Alteration

目的 观察人乳头状瘤病毒 (HPV) 16型和 18型早期蛋白E6及p5 3蛋白在宫颈癌 (CCa)中的表达 ,并分析二者的关系。方法 采用LSAB免疫组化法 ,检测多种宫颈病变中HPV16 .18E6蛋白的表达及CCa中p5 3蛋白的表达。标本包括正常宫颈组织 40例 ,宫颈湿疣 44例 ,宫颈上皮肉瘤变 (CIN)Ⅰ～Ⅱ级 45例 ,CINⅢ级 41例 ,CCa 5 2例。结果 上述宫颈病变组织中HPV16、18E6总阳性率分别为 :2 0 % ,7 1% ,2 2 2 % ,43 9%和 6 7 3% ,各病变组阳性率均显著高于正常宫颈组 (P <0 0 1) ;5 4例CCa中p5 3蛋白阳性者 6例 (11 5 % ) ,均为鳞癌 ,其中 3例伴有HPV16 .18E6阳性。结论 HPV16 .18型感染与CCa及其癌前病变的发生关系密切 ,HPV16 .18E6单克隆抗体可作为检测HPV16 .18感染及CCa早期诊断的标记之一。本研究显示p5

Objective To investigate the expression of human papilloma virus (HPV) 16 and 18E6, and p53 protein in human cervical carcinoma (CCa), and to evaluate relationship between them. Methods LSAB immunohistochemistry was used to detect HPV 16 and 18E6 and p53 proteins in 52 cases of CCa, 41 cervical intraepithelial neoplasia (CIN) III, 45 CIN I～II, 44 cervical condyloma and 40 normal cervixes as control. Results The positive rates of HPV 16 and 18E6 in the above five groups were 67.3%, 43.9%, 22.2%, 7.1% and 2.0% respectively, with a significant difference between the cervical lesions and normal cervix ( P <0.01). Moreover, 6 of the 54 cases of CCa presented high level of p53 protein, of 3 cases showing positivities of HPV16 and 18E6 proteins. Conclusion HPV 16 and 18 infections are closely related with cervical condyloma, CIN and CCa. HPV16 and 18 antibodies could be good indicators for the viruses infection and one of markers for early diagnosis of CCa. This study did not indicate a direct relationship between p53 mutation and HPV16 and 18 infections.