实验性自身免疫性内耳病耳蜗热休克蛋白70的表达

Expression of heat shock protein 70 in immune response of the guinea pig inner ear

目的 探讨自身免疫性内耳病模型动物耳蜗热休克蛋白的表达。方法 利用同种异体内耳抗原免疫豚鼠 ,以建立自身免疫性内耳病 (autoimmuneinnereardisease ,AIED)动物模型 ,并应用免疫组织化学及原位杂位技术 ,研究热休克蛋白 (heatshockprotein ,hsp) 70在正常对照组及AIED模型动物实验组耳蜗中的表达情况。结果 对照组豚鼠螺旋神经节细胞中存在hsp70样蛋白基础表达 ;实验组 2 8耳中 10耳 (35 7% )听阈提高≥ 10dB ;此组动物螺旋神经节细胞和血管纹、螺旋韧带hsp70及其mRNA表达明显增强。结论 以粗制膜迷路抗原免疫豚鼠 ,听阈提高动物hsp70在螺旋神经节细胞和血管纹、螺旋韧带合成增加。

Objective To explore whether the immune response of the inner ear could induce heat shock protein (hsp) 70 in guinea pig cochlea Methods A model of autoimmune inner ear disease (AIED) was established by systemically immunizing the guinea pig with the homologous crude inner ear antigen (CIEAg) The immunized cochleae and normal control cochleae were examined for the expression of hsp70 with techniques of immunohistochemistry and in situ hybridization Results In the control animals, the expression of the hsp70 like protein appeared only in the spiral ganglion, whereas in the cochleae with CIEAg immunization, strong expression of the hsp70 like protein and its mRNA appeared in the spiral ganglion as well as in the stria vascularis and the spiral ligament The hearing thresholds were significantly increased in 10 out of 28 cochleae (35 7%) with CIEAg immunization Conclusion The results suggest that the immune response of the inner ear can induce the expression of hsp70 in the guinea pig cochlea