煤尘致肺泡巨噬细胞氧化损伤的细胞化学研究

IN VITRO CYTOCHEMICAL STUDIES OF FREE RADICAL INJURY ON RAT ALVEOLAR MACROPHAGES BY COAL DUSTS

目的 探讨煤尘致肺泡巨噬细胞 (alveolar macrophages,AM) ,损伤过程中是否存在着氧化损伤机制。 方法 应用细胞化学法显示煤尘作用于 AM产生的 O·2 和 H2 O2 、并做定量测定及相关分析 ,以 Mg2 + - AT-Pase组织化学染色作为检测膜变化的指标。 结果 煤尘致 AM产生的氧自由基较矽尘的少 ,同时细胞的形态结构和膜酶的损伤程度也较低。 结论 (1)应用抗氧化剂可减弱煤尘对 AM所致的损伤 ,进而预防肺成纤维细胞增殖 ;(2 )与矽尘相比 ,煤尘致 AM氧化损伤的程度较弱 ;(3)形态学方法定位显示自由基较之自由基总体测定技术更能直观、具体地阐明细胞损伤的实际状况。

Objective\ To analyze the relationship between the oxygen free radicals and morphological damage of alveolar macrophages(AMs)caused by coal dusts. Methods\ Using morphological technique to demonstrate the oxygen radicals.Moreover,the cytochemical reaction slides of O · 2 and Mg\+ 2+ \|ATPase were analyzed quantitatively by image analyzer,and the linear correlation tendency between the quantitative results of the O · 2 and Mg\+\{2+\}\|ATPase reaction was made. Results\ Like SiO\-2,a toxic mineral particle,exposure of AMs to coal dusts also resulted in production of O · 2 and H\-2O\-2.They were localized on the plasma membrane,the membrane of the phogosome and/or pinosome,and the lysosomal membrane.There is a close relationship between AMs morphological and enzymatic damage and the production of oxygen free radicals. Conclusions\ (1)We can give antioxidant to the patients to terminal oxygenic damage in their bodies,further guard against proliferation of fibroblasts.(2)The degree of oxygen damage induced by coal dusts is lower than that by silica.(3) In situ evaluation of O · 2 and H\-2O\-2 oxygen free radicals is superior to the other techniques in a sense.It is more convenient to directly observe the real situation of the oxidative damage on cells so as to clarify the relationship between the production of free radical and the damage degree on cells.