大豆寡糖对断奶环江香猪小肠发育及SGLT1和GLUT2表达的影响

EFFECTS OF SOYBEAN OLIGOSACCHARIDES ON DEVELOPMENT OF SMALL INTESTINE AND EXPRESSION OF SGLT1 AND GLUT2 IN WEANED HUANJIANG MINI-PIGLETS

目的研究大豆寡糖(SBOS)对小肠发育及其吸收功能的影响。方法选用21 d龄断奶的环江香猪12头,随机分为2组,每组6头,分别饲喂添加0.5%SBOS的日粮和基础日粮,试验期14 d。试验结束后采集血液,肝素抗凝,离心分离血浆,测定血糖浓度;放血处死后取空、回肠组织各两份,另一份采用于组织固定,进行形态学观察;一份利用荧光定量RT-PCR法,测定葡萄糖转运载体SGLT1和GLUT2 mRNA的表达。结果与基础日粮组相比,SBOS组葡萄糖转运载体的表达具有肠道空间位置特异性,空肠SGLT1和GLUT2 mRNA的表达量显著高于回肠(P<0.05);并显著高于基础日粮组空肠的表达量(P<0.05);SBOS显著提高了仔猪小肠绒毛高度与隐窝深度的比值(P<0.05)。结论 SBOS能促进小肠发育,提高肠道对营养物质的吸收。

Ojeetive To investigate the effects of soybean oligosaccharides （SBOS） on development of small intestine and absorption in weaned piglets. Method Twelve Huanjiang mini-piglets weaned at age 21 d were randomly allocated into two groups with six replicates in the feeding trial. The piglets were fed 0% or 0.5% SBOS- supplemented diet for 14 d. The plasma was collected at the end of this trail and used for measuring blood glucose. The jejunum and ileum were collected for histological analysis. The mRNA expression abundances of sodiuml glucose cotransporter （SGLT1） and glucose transporfer （GLUT2） in jejunum and ileum were determined by relative quantitative RT-PCR respectively. Results The mRNA expression of SGLT1 and GLUT2 in SBOS group had intestinal space position specificity, and their expression levels in the jejunum were higher （P〈0.05） than those in ileum, as well as than the control group. SBOS increased （P〈0.05） the ratio of villus height and fossae depth of small intestine Conclusion SBOS can promote development and nutrient absorption ofsmall intestine in mini-piglets.