催眠方煎剂对小鼠海马GABA_A受体mRNA表达的影响

Effects of Cuimian Prescription Decoction on mRNA Expression of GABA_A Receptor in Hippocampus of Mice

目的:研究催眠方煎剂对小鼠海马GABAA受体mRNA表达的影响。方法:实验分为空白对照(等体积蒸馏水)、地西泮(2mg/kg)与催眠方煎剂(2g/kg)组。应用实时荧光定量PCR法检测小鼠海马GABAA受体mRNA的表达。结果:与空白对照组比较,催眠方煎剂组小鼠海马GABAA-α1mRNA表达显著增强,GABAA-α2mRNA表达无显著变化。结论:催眠方煎剂通过特异性作用于GABAA-α1受体而发挥镇静催眠作用。

To study the Cuimian prescription on mRNA expression of GABAA receptor in hippocampus of mice. METHODS： Mice were divided into 3 groups, i.e. blank control group （equal volume of distilled water） group, diazepam （2 rag/ kg） group and Cuimian prescription decoction （2 g/kg） group, mRNA expression of GABAA in hippocampus of mice was detected by RT-PCR techniques. RESULTS： Compared with blank control group, mRNA expression of GABAA in hippocampus of mice increased in Cuimian prescription decoction group. CONCLUSION： Cuimian prescription decoction has edative and hypnotic effects by increasing the gene expression level of GABAA-a receptor.