自制离子液体固相微萃取涂层分析人体尿液中的五氯酚

Determination of Pentachlorophenol in Human Urine by Homemade Ionic Liquid Solid-phase Microextraction Coating

建立了顶空固相微萃取与气相色谱法(HS-SPME-GC)测定人体尿液中五氯酚(PCP)的新方法。采用溶胶-凝胶法,加入自制的离子液体键合固相微萃取涂层,优化了萃取温度、萃取时间、pH值、离子强度及解吸时间。结果表明,样品中加入3 g NaCl,溶液的pH值为2,并以一定速度搅拌的条件下,在80℃顶空萃取50 min,300℃下解吸5 min,方法的检测限为5.0 ng/L,线性范围为0.05～100μg/L,相对标准偏差(RSD)为5.9%,加标回收率为106.6%。

A new method for the determination of pentachlorophenol （PCP） in human urine has been developed by headspace solid-phase mieroextraction with gas chromatography （HS-SPME-GC）. A homemade ionic liquid（IL） bonded coating solid-phase mieroextraetion was added by the sol-gel method. The extraction conditions were optimized as the extraction temperature of 80 ℃, extraction time of 50 min by headspace, desorption time of 5 min at 300 ℃, NaC1 amount of 3 g, pH of 2 and under stirring. The detection limits was 5.0 ng/L. The linear range was 0. 05 - 100 μg/L. The relative standard deviations（RSD） was 5.9%. The recovery was 106. 6%.