^(60)Co-γ辐射对菊苣种子发芽及幼苗生理的影响

Effects of ^(60)Co-γ-rays Irradiation on Chicory Seed Germination and Seedling Physiology

为明确60 Co-γ射线对菊苣(Cichorium intybus L.)辐照诱变效果,采用不同剂量60 Co-γ射线(100Gy,150Gy,200Gy和300Gy)辐照处理菊苣种子,测定其发芽率和相关生理指标。结果表明:随着辐照剂量的增加,菊苣发芽率降低,200Gy以上辐照处理发芽率与对照差异显著(P<0.05);超氧化物歧化酶(SOD)、过氧化物酶(POD)活性、可溶性蛋白和可溶性糖含量随着辐照剂量增加均表现为先升后降的趋势,分别在辐射剂量为150Gy,100Gy,150Gy,150Gy及以上时与对照差异显著(P<0.05);丙二醛(MDA)含量随着辐照剂量增加而逐渐升高,150Gy以上辐照处理与对照差异显著(P<0.05);蛋白含量呈现先增后减的趋势,与对照差异均显著(P<0.05)。由此表明,菊苣受低剂量(150Gy以下)辐照后,诱变效果不明显,高剂量(200Gy以上)辐照下菊苣理化性质发生明显改变,研究结果将为菊苣辐射诱变育种提供一定的理论依据。

Chicory seeds were irradiated with 60Co-γ-rays at 100, 150, 200 and 300 Gy to determine germi- nation rate and physiological characteristics from mutagenic effects of irradiation doses. Results showed that seed germination rate decreased as the irradiation dosage increased. There was significant difference between 200 Gy irradiation dosage and control（P〈0.05）. Superoxide dismutase （SOD） activities, peroxi- dase （POD） activities, soluble protein and soluble sugar contents first increased then declined with a grad- ual irradiation increase, and differed significantly between control and 150 Gy, 100 Gy, 150 Gy, 150 Gy treatments （P〈0.05）, respectively. MDA content increased with increased irradiation dosage, and there was significant difference between control and the treatments above 150 Gy dose （P〈0.05）. The protein content first increased then declined, and differed significantly between control and treatments （P〈O. 05）. It was concluded that the mutagenic effect of chicory irradiated by low dosage （below 150 Gy） was not ob- vious. However, physiological and biochemical characteristics of chicory irradiated above 200 Gy were sig- nificantly altered. The study provides a theoretical basis for chicory radiation mutation breeding.