玉玄颗粒抗炎、祛痰及抑菌作用研究

Study on anti-inflammatory, eliminating phlegm and bacteriostatic action of Yuxuangranule

目的从抗炎、祛痰、抑菌等方面探讨玉玄颗粒治疗咽炎的作用机理。方法采用二甲苯致小鼠耳肿胀、棉球致肉芽肿模型观察其抗炎作用；小鼠酚红排泌实验观察其祛痰活性；体外抑菌试验评价对常见咽炎5种致病菌的抑菌作用；同时测定急性咽炎模型大鼠血常规的变化、血清中肿瘤坏死因子（TNF）、白细胞介素．1（IL．1）水平。结果玉玄颗粒不同浓度组（6．14g／kg、3．07g／kg、1．53g／kg）在急慢性炎症模型中，抗炎作用呈剂量依赖性，在二甲苯诱发小鼠耳胀肿、棉球诱发肉芽肿实验中空白对照组、阳性对照组及高、中、低三个浓度组的肿胀度分别为（9．00±1．50）mg、（3．50±0．10）mg、（4．25±1．08）mg、（5．54±0．97）mg、（8．05±0．08）mg，（1-22±1．50）mg、（0．504-0．10）mg、（0．45±0．08）mg、（0．75±0．97）mg、（0．70±0．08）mg，有明显的祛痰、抑菌作用。对金黄色葡萄球菌、溶血链球菌、肺炎链球菌、伤寒杆菌及白喉杆菌的最低抑菌浓度分别为55、100、68、30、35mg／ml：降低了急性咽炎模型大鼠血浆中白细胞数（9．88±1．22）×109／L、（7．50±1．58）×109／L、（6．02±1．05）×109／L、（8.38±1．13）×109／L、（8．75±1．08）×109／L，中性白细胞（35．62±5．00）×109/L、（28．49±3．40）×109/L、（28．89±5．22）×109/L、（29．34±4．83）×109／L、（33．43±4．27）×109／L，淋巴细胞含量（70．72±5．04）％、（67．89±4．37）％、（60．14±3．88）％、（65．38±4．45）％、（65．65±4．28）％。阳性对照组及玉玄颗粒高、中、低剂量组血清中TNF[分别为（80．23±0．60）ng／ma、（76．83±0．33）ng／ml、（78．00±0．34）ng／ml、（80．52±1．14）ng／ml]明显低于空白对照组（90．25±2．29）ng／mlIL-1水平[分别为149．55±50．02）pg／ml、（130．37±30．61）pg／ml、158．68±40．00）pg／ml、178．764-39．26）pg／ml]均低于空白对照组[（222．06±50．22）pg／mt]。结论玉玄颗粒可能通过抗袭、祛痰、抑菌作用。降低TNF、IL-1水平，达到治疗咽炎的目的。

Objective To reveal the mechanism of Yuxuan granule（YXG） in treating pharyngitis from anti-inflammatory, expectorant, and antibacterial actions. Methods Xylene-induced ear edema and cotton pellet induced granuloma mice models were adopted to observe the anti-inflammatory effects of YXG Phenol red secretion in mice test was adopted to observe the expectorant activity of YXG Five bacteria by tube dilution method was adopted to observe anfimicrobial action of YXG Besides, the changes of routine indexes in blood and tumor necrosis factor （TNF）, interleuldn-1 （IL-1） in serum were measured in acute pharyngitis models. Results YXG has shown a dose-dependent of its anti-inflammatory action. In the test of xylene induced mice ear swelling and cotton ball induced granuloma, the swelling degree of the control group, positive control group and YXG high （6.14g/kg）, medium （3.07g/kg） and low （1.53g/kg） concentration group were （9.00±1.50） mg, （3.50＋0.10）rag, （4.25±1.08）mg, （5.54±0.97）mg, （8.05±0.08）mg and （1.22±1.50）mg, （0.50±0.10）rag, （0.45±0.08） mg, （0.75 ± 0.97） mg, （0.70 ± 0.08） mg respectively. YXG also had obviously expectorant effect to the golden staphylococcus aureus, hemolytic streptococcus, streptococcus pneumoniae, salmonella typhi and corynebacterium diphtheriae, with the minimum inhibition concentration of 55, 100, 68 and 35 mg/ml respectively; Y＇XG also reduced leukocyte count [percentage of leukocyte was （9.88±1.22） × 109/L, （7.50±1.58） × 109/L, （6.02±1.05） × 109/L, （8.38±1.13） × 109/L, （8.75 ±1.08） × 109/[, in the five groups respectively], neutrophils[the value was （35.62±5.00） × 109/L, （28.49±3.40）× 109/L, （28.894445.22） × 109/L, （29.34±4.83） × 109/L, （33.43±4.27） × 109/L in the five groups respectively], lymphocytes [the value was （70.72± 5.04）%, （67.89± 4.37）%, （60.1±3.88）%, （65.38±4.45）%, （65.65±4.28）% in the five groups respectively], TNF in blood serum [the value was （90.25 ± 2.29） ng/ml, （80.23 ± 0.60） ng/ml, （76.83 ± 0.33） ng/ml, （78.00 ± 0.34） ng/ml, （80.52± 1.14）ng/ml in the five groups respectively] and IL-1 [the value was （222.06±50.22）pg/ml, （149.55± 50.02）pg/ml, （130.37 ± 30.61）pg/ml, （158.68 ± 40.00）pg/ml, （178.76 ±39.26）pg/ml in the five groups respectively] in the acute pharyngitis rat. Conclusion Through the anti-inflammatory, expectorant, antimicrobial activity and reducing levels of TNF, IL-1 YXG achieves the goal of treating pharyngitis.