高效毛细管电泳法测定芒果苷在百合知母汤中的含量

Determination of mangiferin in Baihe Zhimu decoction by HPCE

目的建立高效毛细管电泳法测定百合知母汤中有效成分芒果苷的含量。方法采用内标法，选取芦丁为内标，未涂层熔融石英毛细管柱（77cm×75μm，有效长度70cm）为分离通道，以12mmol／L硼砂一甲醇（90：10）为电泳介质，分离电压20kV，检测波长318mm。结果在上述条件下，芒果苷在20min内得到很好的分离，在0．06～1．14mg／ml与峰面积线性关系良好，平均加样回收率为99．0％。结论方法简便快速、结果准确、重复性好，可用于百合知母汤中芒果苷的质量控制。

Objective To establish HPCE method for determining the mangiferin in Baihe Zhimu decoction. Methods Rutin was used as internal standard. An uncoated fused silica capillary （77 cm× 75 μm, effective length of 70 cm） was served as separation gate. The electrolyte buffer was composed of 12 mmoFL borax-MeOH （90 ： 10）. 20 kV voltages were applied and detection wavelength was set at 318nm. Results Mangiferin was successfully separated within 20 rain, the linear response range was 0.06～1.14 mg/ml. The average recovery was 99.0%. Conclusion The method was simple, rapid and well reproducible. It could be used as a reliable tool for the quantity control of Baihe Zhimu decoction and descriptions containing mangiferin.