二氢卟吩e6脂质体的制备及影响因素考察

Preparation and Factors Investigation Affecting Entrapment Efficiency of Chlorin e6 Liposomes

目的:建立二氢卟吩e6脂质体包封率的测定方法,考察各因素对包封率的影响。方法:分别采用Sephadex G50,Sephadex G75凝胶柱分离脂质体和游离药物,优选测定二氢卟吩e6脂质体包封率的方法;以包封率为指标,采用单因素试验考察磷脂质量浓度、胆固醇用量、水化介质、温度及时间对二氢卟吩e6脂质体制备的影响。结果:选择Sephadex G50柱色谱法测定二氢卟吩e6的包封率,其对游离药物回收率达100.6%,采用该法测定包封率的重复性RSD<0.02;磷脂与胆固醇的质量比、药脂比、水化介质对包封率的影响较大。结论:SephadexG50凝胶柱色谱法能方便、快速地测定二氢卟吩e6脂质体的包封率,建立的方法稳定可行。

Objective：To establish a method for determining entrapment efficiency （EE） of chlorin e6 liposomes,and to investigate effect of different factors on it. Method： Sephadex G50 and Sephadex G75 column filtration methods were used to separate free drug and liposomes,in order to optimize a method to determine EE of chlorin e6 liposomes;With EE as index,influence of phospholipid concentration,cholesterol amount,hydration medium,temperature and time on preparation of chlorin e6 liposomes were investigated by single factor test. Result： EE of chlorin e6 liposomes was determined by Sephadex G50 column chromatography,its recovery for free drug was up to 100.6%,RSD of repeatability test was less than 0.02;Ratio of phospholipid-cholesterol,drug-lipid ratio and hydration medium had great effect on EE. Conclusion： Sephadex G50 column filtration method was a good way to determine EE of chlorin e6 liposomes easily and quickly,established method was stable and feasible.