桑枝中桑皮苷A的含量测定

Determination of Mulberroside A in Ramulus Mori by HPLC

目的:建立桑枝中桑皮苷A的含量测定方法。方法:Dikma Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流动相为甲醇-水(15∶85),检测波长324 nm,柱温30℃,流速1 mL.min-1。结果:桑皮苷A在10.34～206.8μg呈良好线性关系(r=0.999 9),平均回收率99.8%;11批桑枝药材中桑皮苷A含量在0.10%～1.69%。结论:此法准确、简便、快速,可用于检测桑枝中桑皮苷A的含量。

Objective：To establish a method for the determination of mulberroside A in Ramulus Mori by HPLC. Method： Dikma Diamonsil C18 column （4.6 mm×250 mm, 5 μm） was used. The mobile phase consisted of methanol and water （15∶85）. The temperature of column was maintained at 30 ℃. The flow rate was 1.0 mL·min-1 and the detection wavelength was set at 324 nm. Result： The calibration curve was in good linearity within the range from 10.34 to 206.8μg （r=0.999 9）. The average recovery was 99.8%. The content of mulberroside A in Ramulus Mori in defferent areas was 0.10%-1.69%. Conclusion： The method is simple, rapid as well as precise and reliable, and can be used for the determination of mulberroside A in Ramulus Mori.