不同生产期苦玄参中苦玄参苷I_A含量测定

Determination of Piefeltarraenin I_A of Picria fel-tarrae in Different Growth Periods

目的:测定同一产地不同生长期的苦玄参药材不同药用部位叶、茎、根中苦玄参苷IA的含量。方法:采用高效液相色谱法(HPLC),C18柱分离分析,以乙腈-0.1%冰醋酸溶液(36∶64)为流动相,流速1 mL.min-1,检测波长264 nm,柱温25℃。结果:苦玄参叶中苦玄参苷IA的平均含量从头年6月份到次年1月份分别为0.37%,0.66%,0.59%,0.45%,0.35%,0.34%,0.31%,0.23%;苦玄参茎中苦玄参苷IA的平均含量从头年6月份到次年1月份分别为0.24%,0.38%,0.36%,0.24%,0.13%,0.08%,0.03%,0.02%;苦玄参根中苦玄参苷IA的含量在7月份最高,为0.18%,其余月份均未检测到。结论:以苦玄参苷IA的含量为参考,苦玄参不同药用部位的苦玄参苷IA均在7,8月份的含量最高,可初步确定苦玄参的最佳采收时间为7,8月份,结果为苦玄参药材的采收加工和规范化种植提供了科学依据。

Objective：To determine the content of piefeltarraenin IA in different medicinal parts of Picria fel-tarrae in different growth periods. Method： High performance of liquid chromatography （HPLC） was adopted, the separation was performed on C18 column with acetonitrile-0.1% aqueous acetic acid solution （36∶64） as mobile phase, the flow rate was 1 mL·min-1, the detection wavelength was set at 264 nm and the column temperature was kept at 25 ℃. Result： From the last year in June to the next year in January, average content of piefeltarraenin IA in leaves were listed monthly as follows： 0.37%, 0.66%, 0.59%, 0.45%, 0.35%, 0.34%,0.31%, 0.23%; and in the stems： 0.24%, 0.38%, 0.36%, 0.24%, 0.13%, 0.08%, 0.03%, 0.02%; the content of piefeltarraenin IA in roots was 0.18% in July and was not detected in other times of the whole year. Conclusion： The content of piefeltarraenin IA was highest in July and August, regarded the content of piefeltarraenin IA as standard, the optimum collection time of Picria fel-tarrae was in July and August. This research could provide scientific basis for optimizing the collection time and actualizing standard planting of Picria fel-tarrae.