HPLC测定壮药马蹄金中伞形花内酯的含量

Determination of Umbelliferone in Dichondra repens of Traditional Zhuang Medicine

目的:建立马蹄金中伞形花内酯的含量测定方法。方法:采用VP-ODS C18(4.6 mm×150 mm,5μm)色谱柱,以甲醇-四氢呋喃水溶液(15∶85)为流动相,流速1.0 mL.min,检测波长325 nm。结果:马蹄金中的伞形花内酯含量测定方法在0.050 7～0.507 0μg时进样量与峰面积的线性关系良好(r=0.999 8),平均回收率98.85%,RSD 1.00%。结论:所建立的方法准确、灵敏、重复性好,可用于马蹄金的质量控制。

Objective：To establish the determination method of umbelliferone content in Dichondra repens. Method： VP-ODS Cl8（4.6 mm×150 mm, 5 μm） column was used. The mobile phase was methanol-tetrahydrofuran-water solution （15∶85）; the flow rate was 1.0 mL·min-1; the detection wavelength was at 325 nm. Result： The linear relation of hyperin was good in the range from 0.050 7 to 0.507 0 μg. The average recovery was 98.85%, RSD was 1.00%. Conclusion： The method established is accurate, sensitive and good in reproducibility. It can be applied to the quality control of D. repens.