银杏叶提取物中银杏萜内酯成分大气压化学电离质谱分析

Atmospheric Pressure Chemistry Ionization Mass Spectrum Analysis of Gingkolides in Ginkgo biloba Extract

目的:用高效液相色谱-大气压化学电离-质谱(HPLC-APCI-MS)法对银杏叶提取物中银杏内酯进行分析。方法:液相色谱条件Agilent 3000 Extend-C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-水30∶70,流速0.8 mL.min-1,检测波长220 nm,柱温35℃;负离子模式检测。结果:解析鉴别了白果内酯和银杏内酯A,B,C,J,其分子离子峰的m/z分别为324.8,407.1,422.9,438.9,422.9。结论:为银杏内酯的检测提供了一种快速有效的质谱方法。

Objective：To study gingkolides in Ginkgo biloba extract by HPLC-APCI-MS. Method： Analysis of gingkolides was done by HPLC-APCI-MS in negative ion mode. The separation was performed on Agilent 3000 Extend-C18 column with methanol-water （30∶70） as mobile phase, and the flow rate was 0.8 mL·min-1. The spectrometer was APCI-MS. Result： Bibobalide, ginkgolide A,B,C,J were identified, the m/z of molecular ion peak is respectively 324.8,407.1,422.9,438.9,422.9. Conclusion ：The established method by HPLC-APCI-MS can be adopted for sensitive and rapid identification of gingkolides.