摘要
目的:用高效液相色谱-大气压化学电离-质谱(HPLC-APCI-MS)法对银杏叶提取物中银杏内酯进行分析。方法:液相色谱条件Agilent 3000 Extend-C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-水30∶70,流速0.8 mL.min-1,检测波长220 nm,柱温35℃;负离子模式检测。结果:解析鉴别了白果内酯和银杏内酯A,B,C,J,其分子离子峰的m/z分别为324.8,407.1,422.9,438.9,422.9。结论:为银杏内酯的检测提供了一种快速有效的质谱方法。
Objective:To study gingkolides in Ginkgo biloba extract by HPLC-APCI-MS. Method: Analysis of gingkolides was done by HPLC-APCI-MS in negative ion mode. The separation was performed on Agilent 3000 Extend-C18 column with methanol-water (30∶70) as mobile phase, and the flow rate was 0.8 mL·min-1. The spectrometer was APCI-MS. Result: Bibobalide, ginkgolide A,B,C,J were identified, the m/z of molecular ion peak is respectively 324.8,407.1,422.9,438.9,422.9. Conclusion :The established method by HPLC-APCI-MS can be adopted for sensitive and rapid identification of gingkolides.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第5期155-158,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家山东创新药物孵化基地项目(2010ZX09401-0302-4-11)
关键词
银杏叶提取物
银杏内酯
高效液相色谱
质谱
大气压化学电离
Ginkgo biloba extract
gingkolides
HPLC
mass spectrometer
atmospheric pressure chemistry ionization