胶质芽孢杆菌液体发酵产孢条件的优化

Culture conditions optimization in submerged fermentation of Bacillus mucilaginous

以提高胶质芽孢杆菌液体发酵产孢量为目的,以血球计数板计数芽孢数量,以产孢量为衡量指标,设计单因素实验和正交实验,对胶质芽孢杆菌液体发酵培养条件进行优化研究。优化后的发酵工艺参数为:接种量5%,起始pH7.5,碳酸钙用量6g/1000mL,装液量70mL/250mL三角瓶,转速250r/min,发酵前24h温度为32℃,然后升高温度到37℃,继续发酵12h。按优化条件培养做生长曲线进行验证,培养36h血球计数板计数芽孢数量为9.3×109cfu/mL。

To improve the spore yeild of Bacillus mucilaginous in submerged fermentation,optimizing process of the culture conditions including single factor experiments and orthogonal experiment was carried out and spore yeild according to blood cell counts board was used as an indicator. The results showed that the optimal culture conditions were： inoculation amount of 5%, initial pH of 7.5, CaCO3 amount of 6g/1000mL, broth volume of 70mL/250mL flask, rotate speed of 250r/min,temperature of 32℃ for the beginning 24h,then increased to 37℃ for 12h further, and incubation time of 36h totally. Under the optimal culture conditions, the spore yeild of Bacillus mucilaginous was 9.3×10^9cfu/mL at 36h using blood cell counts board.