白酒酒醅中纤维素酶产生菌的产酶活力与其生理生化特性的研究

Study on cellulase activity production and physiological and biochemical characteristics of cellulase producing bacteria isolated from wine fermented grains

以白酒酒醅中筛选出的产纤维素酶的细菌为研究对象,利用相关分析和通径分析研究了纤维素酶产生菌的生理生化特性与其产酶活力之间的关系。相关分析和通经分析结果表明:细胞长宽比和葡萄糖产酸指标与纤维素酶活力具有明显的负相关性;淀粉水解实验指标与纤维素酶活力具有明显的正相关性;细胞长宽比对细菌产纤维素酶的负向直接作用最大;葡萄糖产酸指标对纤维素酶活力有较强的负向直接作用;葡萄糖产气和酪蛋白实验指标对纤维素酶具有较强的正向直接作用;同时各个指标还通过其他指标对纤维素酶活力具有间接作用。

A correlation between the physiological and biochemical characteristics of cellulase producing bacteria isolated from wine fermented grains and cellulose enzyme activity was studied using correlation analysis and path analysis.Results of correlation analysis and path analysis showed that cell aspect ratio and acid production indicator using glucose had clear negative correlations with cellulase activity,while starch hydrolysis test indicator had positive correlation with cellulase activity.Cell aspect ratio had the largest negative direct effect on cellulase activity.Acid production indicator using glucose had strong negative direct effect on cellulase activity. Gas production indicator used glucose and casein test indicator had strong positive direct effects on cellulase activity, as well as individual indicators also had indirect effects on cellulase activity through other indicators.