摘要
为确定红雪茶类胡萝卜素的最佳提取工艺条件,在单因素实验的基础上,选择丙酮为浸提溶剂,料液比、提取温度、提取时间作为自变量,以类胡萝卜素特定波长下吸光度值作为响应值,基于Box-Behnken中心组合方法进行三因素三水平的实验设计,根据响应面结果,建立了回归模型。研究结果表明,红雪茶类胡萝卜素的最优提取工艺条件参数为:料液比1∶3(g/mL),提取温度52℃,提取时间20min。在该条件下,红雪茶类胡萝卜素提取液的吸光值为0.3682,实际获取红雪茶类胡萝卜素量22.092μg/g,与预测值相对误差较小。说明该模型拟合度好,优化后得到的提取条件准确、可靠。
Based on Box-Behnken center composite design within Design-Expert7.0 software and response surface results,the optimal extraction process for carotenoids from Lethariella spp.were determined and a regression model was established.On the basis of single factor tests,using absorbance values in a specific wavelength of carotenoids as response value, three key factors (solid- liquid ratio, extraction temperature and time)were selected as independent variables and acetone as extraction solvent.The results showed that the optimal extraction process of carotenoids from Lethariella spp.was obtained as followed.solid-liquid ratio of 1:3 (g/mL) ,extraction temperature of 52℃,and extraction time of 20min. Under these conditions, the extraction of carotenoids from Lethariella spp. absorbance was 0.3682, i.e.22.092μg from per g Lethariella spp. raw materials.The results showed that regression equation fit well with experimental data because of a good accordance between the predicted and observe values. Meanwhile the optimized technology was stable and viable with minor error and good degree.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第5期184-188,共5页
Science and Technology of Food Industry
基金
国家自然科学基金项目(31060031)
云南省教育厅基金一般项目(2010Y433)
云南省本科教学实验生物技术示范中心项目(0205-2001015209)
云南民族大学青年科学基金(09QN05)
关键词
天然色素
红雪茶
类胡萝卜素
响应面分析
natural pigments
Lethariella spp.
carotenoids
response surface analysis