摘要
为准确检测贝类邻苯二甲酸酯(PAEs)总量,通过响应面法优化荧光法测定样品中PAEs的条件。探讨了不同NaOH浓度,缓冲溶液pH,EDTA-Fe(Ⅱ)和H2O2用量对PAEs在Fenton反应体系中产生荧光强度的影响。经响应面优化的最佳荧光反应条件如下:NaOH溶液的浓度为4.61mol/L,缓冲溶液的pH为7.09,EDTA-Fe(Ⅱ)和H2O2溶液的加入量分别为0.55和1.63mL。螺蛳样品采用正己烷与二氯甲烷1∶1混合溶液按1∶1.6的固液比超声振荡萃取后在优化条件下测定PAEs总量,方法的相对标准偏差为1.73%~6.56%,加标回收率为93.80%~106.11%,所检测螺蛳中PAEs含量达26.64~44.89mg/kg。
The optimum conditions for determining total phthalate esters(PAEs) in snail samples by fluorimetry were established by using response surface methodology( RSM).The optimal levels were as follows:the concentration of NaOH was 4.61 mol/L, the pH of Na2 HPO4- Nail2 PO4 buffer solution was 7.09, and the volume of EDTA- Fe (II ) and H2O2 was 0.55mL, 1.63 mL respectively.Snail samples were ultrasonic extracted by hexane and dichloromethane 1:1mixed solution with 1:1.6 (g/mL)ratio of solid-to-liquid at first, and then were performed under the optimized conditions as mentioned above.The results showed that the relative standard deviation of the method were 1.73% 6.56%,the average recoveries ranged from 93.80% to 106.11%, and the PAEs of snail was about 26.64 44.89mg/kg.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第5期304-308,共5页
Science and Technology of Food Industry
基金
浙江省公益性技术应用研究计划项目(2011C37068)
关键词
邻苯二甲酸酯
荧光法
响应面
螺蛳
phthalate esters
fluorimetry
response surface methodology
snail