高效液相色谱法测定铁皮石斛中柚皮素的含量

Determination of naringenin in Dendrobium officinale by HPLC

目的:探寻铁皮石斛中指标性成分,建立指标性成分的含量测定方法,并测定浙江省不同种植基地、不同生长年限铁皮石斛中柚皮素的含量。方法:采用高效液相色谱法测定柚皮素的含量。色谱柱为XB C18(4.6 mm×250 mm,5μm),以甲醇-0.2%磷酸水溶液为流动相,检测波长为290 nm。结果:柚皮素在0.026～0.208μg线性关系良好(r=1);平均加样回收率为96.3%(RSD 1.8%)。同一生长年限不同种植基地铁皮石斛中柚皮素含量波动范围较大;并且同一种植基地铁皮石斛随着生长年限的增加柚皮素的含量先增加后降低,三年生铁皮石斛中柚皮素的含量最高。结论:该方法准确、可靠,可用于铁皮石斛药材及相关产品中柚皮素的含量测定,为完善铁皮石斛药材质量标准提供参考。

Objective ： To explore a characteristic chemical marker of Dendrobium officinale, establish determination method of its content and determine the naringenin content in D. officinale from different sources and growth years. Method： The content of naringenin was determined by HPLC. HPLC analysis was made on a XB -C18 （4. 6 mm ×250 mm, 5 μm） with methanol and water containing 0. 2% phosphoric acid as mobile phase. The detection wavelength was 290 nm. Result： The HPLC method showed good linearity within .the range of 0. 026-0. 208 μg （ r = 1 ）. The average recovery of naringenin was 96. 3% （ RSD 1.8% ）. The naringenin content was the highest in 3 years D. officinale and had some differences from different sources. Conclusion： The method is accurate and reliable. It is appropriate for the quantitative determination of naringenin in D. officinale and it＇s production.