超高压处理对鲜人参微生物及人参皂苷含量的影响

Effect of ultra high-pressure processing on microorganisms and ginsenosides of Panax ginseng

目的:通过比较鲜人参超高压处理前后细菌和霉菌的菌落总数和人参皂苷含量的变化,探讨超高压处理技术作为鲜人参防腐防霉方法的可行性。方法:采用微生物学检验法测定细菌总数和霉菌总数;HPLC测定12种主要人参皂苷的含量。结果:在所选择的3组条件下,超高压处理后鲜人参菌落总数显著下降,霉菌未被检出;人参甲醇提取物和水提取物中12种人参皂苷的含量明显提高。结论:超高压处理鲜人参不但可以防腐防霉,而且提高人参皂苷的浸出率,是一种值得进一步深入研究的潜在的高效、安全和环保的人参防腐防霉技术。

Objective： To study the feasibility of the application of ultra high-pressure processing （UHPP） as an anticorrosion and anti-mould method by comparing the total numbers of bacteria and mould colonies and the content of ginsenosides before and after UHPP. Method： The total numbers of bacteria and moulds colony were determined by microbiological test method. The contents of 12 ginsenosides were determined by HPLC. Result： Under the three selected conditions, the total number of bacterial colony decreased significantly,while the mould was not detected in UHPP samples; and the contents of 12 ginsenosides were increased significantly in methanol extracts and water extracts. Conclusion： UHPP not only shows anticorrosion and anti-mould effects, but also enhances the leaching rate of ginsenosides. It is a highly effective, safe and environmental friendly anticorrosion and anti-mould technique for Ranax ginseng worth in-depth study.