生物强化接触氧化工艺原位修复受氮素污染水体

Bioaugmentation of Biological Contact Oxidation Process for Remediation of Nitrogen-polluted Water

某受氮素污染河水采用接触氧化工艺处理但效果不理想,为此,模拟该河水真实流态而构建了小试装置,通过功能菌群调查,富集、驯化培养土著氨氧化菌(AOB)和亚硝酸盐氧化菌(NOB)并持续投加至好氧段,以强化硝化菌群、提高脱氮效果。采取生物强化措施后,系统对总氮和氨氮的去除率分别提高了约26%和20%;出水中的AOB和NOB菌群数量分别增加了近103和104倍,好氧段生物膜中的AOB和NOB菌群数量均增加了近102倍。DGGE图谱分析也表明,投加高效菌剂后,系统中的微生物群落结构发生了明显变化,新增的优势微生物种群与Nitrosomonassp.和Nitrobacter sp.具有高度同源性,说明系统内去除氮素的关键微生物种群得到了增强。

A small-scale test device was designed to simulate the actual flow pattern of a nitrogenpolluted river due to low treatment efficiency of biological contact oxidation process. Indigenous ammoniaoxidizing bacteria （AOB） and nitrite-oxidizing bacteria （NOB） were domesticated, cultivated and added to the aerobic zone in order to enhance nitrifier population and improve nitrogen removal efficiency. After bioaugmentation, the removal rates of TN and NH4^＋ - N increased by 26% and 20% , respectively. The numbers of AOB and NOB in effluent increased by 103 and 104 times respectively, and those in biofilm both increased by 102 times. DGGE profiles revealed that the bacterial community structure obviously changed, and there was high homology between the added predominant bacterial communities and the species of Nitrosomonas and Nitrobacter.