HSR反应时间对HSR-SBR系统除磷效能的影响

Effect of Reaction Time of Humus Soil Reactor on Phosphorus Removal Performance in Side-stream Humus Soil Sequencing Batch Reactor

考察了腐殖土反应器(HSR)的反应时间分别为12、24和48 h的3组侧流腐殖土SBR系统(HSR12-SBR1、HSR24-SBR2和HSR48-SBR3)的除磷效果。结果表明,3组系统对TP的去除率分别为(64.5±24.0)%、(85.6±9.8)%和(93.6±8.0)%,HSR48-SBR3的除磷效果明显高于HSR24-SBR2和HSR12-SBR1。通过荧光原位杂交技术(FISH)分析发现,SBR1、SBR2和SBR3中聚磷菌(PAOs)的含量分别为(20.8±6.9)%、(62.9±15.1)%和(53.6±4.9)%;聚糖菌(GAOs)的含量分别为(47.9±8.5)%、(27.4±2.3)%和(27.9±9.7)%。进一步测定PAOs的除磷相关酶活性发现,SBR1、SBR2和SBR3中PAOs的聚磷酸盐激酶(PPK)活性分别为(0.687±0.014)、(0.838±0.037)和(1.061±0.013)μmolNADPH/(min.mg protein),PPK活性在HSR反应时间为48 h时显著增强,提高了PAOs好氧阶段过量吸磷的能力。综上可知,增加HSR的反应时间可改善微生物种群结构,增强PAOs的PPK活性,提高系统的除磷效果。

Lab-scale side-stream humus soil sequencing batch reactors （HSR12-SBR1, HSR24-SBR2 and HSR48-SBR3） were operated continuously to investigate the influence of reaction time （ 12, 24 and 48 h） of humus soil reactors on the phosphorus removal efficiency. The results showed that the average phosphorus removal efficiencies of HSR12-SBR1, HSR24-SBR2 and HSR48-SBR3 were （64. 5 ± 24.0） %, （85.6 ± 9.8） % and （93.6± 8.0） %, respectively. Fluorescence in situ hybridization technique revealed that PAOs in SBR1 , SBR2 and SBR3 accounted for （20.8 ± 6.9） %, （62.9 ±15.1 ） % and （53.6±4.9） % of the total biomass, respectively ; and GAOs accounted for （47.9 ± 8.5 ） %, （27.4 ± 2.3 ）% and （27.9 ±9.7 ）% of the total biomass, respectively. The polyphosphate kinase activitiesofPAOs in SBR1, SBR2 and SBR3 were （0.687±0.014）, （0.838±0.037） and （1.061 ± 0. 013 ） μmolNADPH/（min·mg protein）, respectively. The polyphosphate kinase activity was the highest at the reaction time of 48 h, enhancing the phosphate uptake ability of the PAOs in the aerobic stage. Increasing reaction time could improve microbial community structure, enhance polyphosphate kinase activity, thus improving the phosphorus removal efficiency.