芦丁锗的合成、表征及生物活性研究

Study on Synthesis,Characterization and Biological Activities of Rutin-germanium

芦丁在碱溶液中,与氧化锗反应生成芦丁锗;通过UV、IR、1H NMR,确定了锗离子与芦丁的螯合位置;通过差热分析,确定了配合物中结晶水的数量和锗的含量;通过四甲基偶氮唑蓝(MTT)法和抗氧化性实验,测定了芦丁锗的生物活性。结果显示:芦丁锗对人子宫颈癌Hela细胞、肺癌SPC-A-1细胞及人食管癌EC9706细胞均没有抗肿瘤活性;清除DPPH自由基实验显示,芦丁锗较芦丁具有更强的清除自由基能力。

Rutin-germanlum was synthesized from turin and germanium in the alkaline solution and the chelation position was characterized by UV ,IR and I H NMR. The number of the crystal water and the content of germanium in the complex were determined using thermal analysis. Methyl thiazolyl tetrazelium blue （MTF） experiments on the human cervicalca neer Hela cell,lung cancer SPC-A-1 cells and human esophageal cancer EC9706 cells proved that rutin-germanium had no anti-tumor activity. DPPH radical scavenging experiments showed that the radical scavenging capacity of rutin-germa- nium was stronger than rutin.