摘要
本文对分离自海洋样品具有神经氨酸酶抑制活性的放线菌FIM09-1157进行初步的分类研究,其形态特征和16S rDNA序列分析表明该菌属于链孢囊菌属(Streptosporangium sp.)。采用硅胶柱色谱、凝胶层析以及高效制备液相对链孢囊菌FIM 09-1157发酵代谢产物进行分离纯化,获得活性化合物T1。理化性质和UV、MS以及1D和2D-NMR波谱分析结果表明化合物T1与链霉菌产生的硫内酯类化合物Thiotetromycin同质。本文首次报道了化合物T1体外神经氨酸酶抑制活性,其IC50值为92μmol/L。
Based on morphological characteristics and 16S rDNA sequence analysis, actinomycete strain FIM09-1157, which was isolated from marine sediment,was classified as Streptosporangium sp. The bioactive metabolite T1 was isolated and purified from the fermentation broth of Streptosporangium FIM09-1157 using silica gel chromatography, gel chromatography and high performance preparative chromatography. Basic physico-chemical properties and UV, MS and 1D, 2D-NMR spectral data revealed that compound T1 was identical with thiolactune-containing antibiotic thiotetromycin. It was the first report for compound T1 to show potentlalantineuraminidase activity with ICs0 value of 92 mol/L in vitro
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2013年第2期193-196,共4页
Natural Product Research and Development
基金
福建省科技计划重点项目(2011R1010-4和2011R1010-2)
国家科技重大专项“重大新药创新”(2010ZX09401-403,2012ZX09301002-003)
