蛇床子素预处理对内毒素致小鼠急性肺损伤的影响

The effect of osthol on acute lung injury induced by LPS in mice

目的探讨蛇床子素（Ost）预处理对脂多糖（LPS）引起的急性肺损伤的防治作用并初步研究其作用机制。方法BALB／C小鼠随机分为生理盐水对照组（NS组）、蛇床子素对照组（Ost组）、模型组（LPS组）及蛇床子素处理组（Ost＋LPS组）。气管滴注LPS建立小鼠急性肺损伤模型。造模6h后测定肺湿／干质量比值（W／D）、肺泡灌洗液（BALF）中蛋白含量及肿瘤坏死因子（TNF-a）浓度，同时观察肺组织病理学改变。结果LPS可明显提高小鼠肺W／D、BALF中蛋白含量和TNF-a浓度（P〈0．05），肺组织结构发生明显的病理改变；Ost处理组可有效减轻LPS引起的上述变化（P〈0．05），组织损伤程度明显减轻。结论Ost预处理对LPS导致的小鼠急性肺损伤有保护作用，其保护机制可能与抑制LPS诱导的肺通透性增加有关。

Objective To investigate the prevention effect of osthol on acute lung injury （ALI） induced by LPS in mice and its potential mechanism. Methods BALB/C mice were randomly divided into four groups ： saline control group, osthol group, LPS group and STS ＋ LPS group, the animal model of ALI was established by intratracheal instillation of LPS（5 mg/kg）. 6 h after intratracheal instillation of LPS, the left lung wet - to - dry （ W/D ） ratios, protein content and the level of tumor necrosis factor - alpha （ TNF - ot ） in bronchoalveolar lavage fluid （BALF） were measured, Meanwhile, the histological changes of lungs were observed. Results The W/D, protein content and the level of TNF - et in BALF were highly.increased in LPS group（ P 〈 0.05 ）, histological studied showed that there were congestion, edema and the sequestration of inflammatory cells in lung tissues in LPS group, but pretreatment of osthol can inhibit the increased parameters（ P 〈 0.05 ） and lung injury was significantly alleviated. Conclusion Osthol could prevent mice ALI induced by LPS, and the mechanism of protection was correlated with inhibition of lung permeability.