摘要
采用化学共沉淀法制备出Fe_3O_4纳米颗粒,将其加入至正硅酸乙酯(TEOS)的凝胶体系中,制备得到Fe_3O_4-SiO_2复合颗粒,再通过硅烷化试剂3-氨丙基三乙氧基硅烷(APTES)对其进行硅烷化修饰,得到表面氨基功能化的磁性纳米微球。X射线分析仪、傅里叶红外光谱仪、振动样品磁强计、透射电子显微镜等分析表明,制备出的氨基硅烷化磁性微球具有良好的超顺磁特性,粒径约为25nm。与游离纤维素酶相比,利用该磁性微球固定化纤维素酶,固定化酶的热稳定性明显提高并具有良好的重复使用性能。
Magnetic nanoparticles were synthesized by chemical co-precipitation Fe2＋ and Fe3＋ in ammonia solu- tion. Amino-silane modified Fe3O4 coated with SiO2 were prepared via sol-gel process and silanization reaction. The morphology and properties of these magnetic nanoparticles were examined by transmission electron microscopy （TEM）, X ray diffraction experiment（ XRD）, Fourier transform infrared spectroscopy（FTIR） and vibrating sample magnetometer（VSM）. The results indicated that Fe3O4 magnetic nanoparticles with an average diameter of 25nm were supermagnetic and well coated by SiO2. Compared with free cellulase, the thermal and operational character- istics of the immobilized cellulase were increased after immobilization with the prepared microsphere.
出处
《太阳能学报》
EI
CAS
CSCD
北大核心
2013年第2期337-342,共6页
Acta Energiae Solaris Sinica
基金
国家科技支撑计划(2011BAD22B01)
国家自然科学基金(21176237
21211140237)
中科院-广西科学院院地合作项目
关键词
纳米FE3O4
硅烷化修饰
纤维素酶
固定化
magnetic nanoparticles
amino-silane modification
cellulase
immobilization
