摘要
为了构建能同时发酵葡萄糖和木糖的酵母重组菌株,采用双灭活原生质体技术,通过聚乙二醇将树干毕赤酵母(Pichia stipitis)NRRL Y-7124与酿酒酵母(Saccharomyces cerevisiae)NRRL Y-2034进行融合,选育出一株遗传性能稳定且发酵木糖产乙醇性能显著提高的优良融合菌株F11。融合子F11在60g/L木糖中发酵,乙醇产量较亲本提高30.27%,达到20.09g/L;在60g/L混合糖(葡萄糖:木糖=1:3)发酵中,融合子F11的乙醇最高产量为22.52g/L,比亲本提高29.7%,同时融合子的耐乙醇能力也明显增强。
To construct glucose and xylose co-fermenting yeast, protoplasts of Pichia stipitis NRRL Y-7124 and Saccharomyces cerevisiae NRRL Y-2034 were both inactivated, followed by the PEG-mediated protoplast fusion. A fusant Fll was screened with high genetic stability and xylose-fermenting ability. Fusant Fll can produce 20.09g/L and 22.52g/L ethanol from 60g/L xylose and 60g/L mixed sugar（ glucose ： xylose = 1 ： 3 ）, respectively. Compared with the parent Pichia stipitis NRRL Y-7124, the highest ethanol concentration was increased by 30.27% and 29.7%, respectively. Additionally, the ethanol tolerance of Fll is much better than that of the parent P. stipitis. The successful construction of fusant F11 lays the foundation for further sufficient fermentation of all the sugars found in lignocellulosic hydrolysates to ethanol.
出处
《太阳能学报》
EI
CAS
CSCD
北大核心
2013年第2期343-348,共6页
Acta Energiae Solaris Sinica
基金
国家高技术研究发展(863)计划(No.2009AA05Z436)
中科院广州能源研究所所长创新基金人才引进项目(No.o807rd)
关键词
树干毕赤酵母
酿酒酵母
原生质体融合
发酵
Pichia stipitis
Saccharomyces cerevisiae
protoplast fusion
fermentation
