乙二醇壳聚糖接枝羧甲基β-环糊精用于运载疏水性抗癌药物甲氨蝶呤

Glycol chitosan-graft-carboxymethyl β-cyclodextrin as carrier of hydrophobic anticancer drug methotrexate

利用简单的交联反应合成乙二醇壳聚糖接枝羧甲基β-环糊精(GCH-g-CMβ-CD),通过红外光谱、核磁共振谱和浓硫酸-苯酚降解法对其进行表征.将GCH-g-CMβ-CD用于运载疏水性抗癌药物甲氨蝶呤(MTX),应用表面等离子体激元共振仪在线原位监测其与MTX的结合,紫外吸收分光光度法测定主客体分子的结合比为1∶1.证明GCH-g-CMβ-CD通过CMβ-CD的疏水空腔与MTX结合,形成稳定的水溶性复合物.因此,GCH-g-CMβ-CD有望用于疏水性抗肿瘤药物的运载.

Glycol chitosan-graft-carboxymethylfl-cyclodextrin （GCH-g-CM fl-CD） was synthesized by a simple cross-linking reaction, and the products were characterized by FT-IR,13 C-NMR, and HESO4-phenol degradation method. Interaction between GCH-g-CM β-CD and hydrophobic anticancer drug methotrexate （MTX） was studied by surface plasmon resonance （SPR） in real time and in situ. UV-vis spectroscopy analysis indicated that each CM β-CD unit binds one MTX molecule, which showed that MTX can be successfully loaded into the hydrophobic cavities of grafted CM β-CDs. The results indicated that the newly synthesized GCH-g-CM fl-CD can be used as hydrophobic anticancer drug carrier.