异型流感病毒感染小鼠T细胞亚群变化研究

Changes in T lymphocyte subsets of mice infected with the heterosubtypic influenza virus

目的制备能够抵御不同类型流感病毒感染疫苗,揭示机体对异型流感病毒感染交叉免疫保护作用的主要机制。方法小鼠流感病毒疫苗免疫后分别感染同型、异型流感病毒,另设使用免疫增强剂IL-2后感染异型流感病毒组,观察小鼠的一般状况和肺指数,并用流式细胞术检测小鼠肺和脾中的CD4+、CD8+T细胞水平。结果异型免疫组和异型免疫加强组流感病毒感染后小鼠肺和脾中CD4+、CD8+T细胞百分率显著高于感染前及同型免疫组和对照组(P<0.05)。病毒感染后7d,同型免疫组和异型免疫加强组小鼠存活率均为100%,异形免疫组为67%。结论小鼠感染异型流感病毒后CD4+、CD8+T细胞水平显著增高,此细胞可能在流感病毒异型间交叉保护免疫应答反应中起重要作用,其机制有待进一步研究。

Objective To prepare vaccines against the different subtypes of influenza virus and reveal the main mecha- nism of cross-protective immunity from heterosubtypic influenza virus infection. Methods Mice immunized with influ- enza virus vaccine were infected with the same type and heterosubtypic influenza virus, and a group of mice were injected with IL-2 as an immunostimulant after heterosubtypic influenza virus infection. The general condition and lung index of mice were observed, lung homogenates were collected, and the CD4＋ , CD8＋T cell levels were detected in the lungs and spleen using flow cytometry. Results The percentage of CD4＋ , CD8＋ T cells in the lungs and spleen was significantly higher after heterosubtypic influenza virus infection （P-~0.05） compared to mice infected with the same type of influenza virus or mock-infected. Seven days after influenza virus infection, the group infected with the same type and the group u sing an immunostimulant both had a survival rate of 100~ ; the group infected with only the heterosubtypic influenza virus had a survival rate of 67~. Conclusion The percentage of CD4＋ , CD8＋T ceils significantly increased after heterosub typic influenza virus infeetion, and these cells may play an important role in immunity against heterotypic influenza virus infection. The mechanism of this increase in lymphocytes must be studied further.