2008～2010年青海省流行性感冒病毒监测结果分析

Analysis of the results of monitoring influenza virus in Qinghai from 2008 to 2010

目的对2008～2010年青海省流感病毒监测结果进行分析,及时发现变异病毒和新型毒株,预测流感流行趋势。方法采用Real-time PCR方法进行流感病毒检测,采用MDCK细胞培养法分离流感病毒。结果 2008～2010年青海省收集监测标本9 882份,分离流感病毒2 347株,分离率23.75%;2008年分离64株流感病毒,其中H1N1亚型56株,H3N2亚型6株,B型2株;2009年检出新甲型H1N1阳性2 034份,分离流感毒株58株,其中H1N1亚型31株,H3N2亚型17株,B型10株;2010年检出病毒218株,其中季节性H1N1亚型4株,季节性H3N2亚型140株,B型61株,甲型H1N1 13株。结论 2008年季节性H1N1亚型为优势株,2009年优势株为甲型H1N1流感,2010年为H3N2亚型。

Objective To analyze influenza viruses by monitoring influenza in Qinghai Province from 2008 to 2010 in order to discover variations in viruses and new subtypes and predict trends in influenza outbreaks. Methods Real-time PCR was used to detect influenza viruses and MDCK cell culturing was used to isolate influenza viruses. Results Nine thousand eight hundred and eighty-two specimens were collected in Qinghai Province from 2008 to 2010, and 2,347 strains of influenza viruses were isola- ted from these specimens at a rate of 23.75%. In 2008, 64 strains of influenza viruses were isolated; of these, 56 were strains of H1N1, 6 were strains of H3N2, and 2 were strains of influenza B. In 2009, 2,034 specimens tested positive for AH1N1 accord- ing to real-time PCR and 58 strains were isolated： 31 strains of H1N1, 17 strains of H3N3, and 10 strains of influenza B. In 2010, 218 strains were detected： 4 were strains of H1 N1, 61 were strains of influenza B, 140 were strains of H3 N2, and 13 were strains of AH1N1. Conclusion The predominant strain of influenza was H1N1 in 2008. In 2009, the predominant strain was AHIN1. In 2010, the predominant strain was H3N2.