肠易激综合征血循环microRNA的表达谱

Expression prof ile of circulating microRNAs in patients with irritable bowel syndrome

目的:探讨腹泻型肠易激综合征(irritable bowel syndrome,IBS)、便秘型IBS和正常人体血循环中微小核糖核酸(microRNA,miRNA)的表达差异,寻找潜在的IBS异常miRNA表达谱.方法:按罗马Ⅲ标准临床诊断IBS,根据临床特点分为腹泻型IBS和便秘型IBS.分别选取3份腹泻型IBS、3份便秘型IBS和3份正常人的混合血清标本,miRNA表达芯片检测血清标本中miRNA的表达水平,并进行聚类分析.结果:miRNA表达芯片分析发现,与正常人相比较,腹泻型IBS血循环中2种miRNA表达下调,2种miRNA表达上调;便秘型IBS血循环中4种miRNA表达下调,59种miRNA表达上调;只在腹泻型IBS血循环中差异表达的miRNA有1种,只在便秘型IBS血循环中差异表达的miRNA有60种,miR-23b在两种IBS血循环中都表达显著下调,hcmv-miR-US5-2、hsv2-miR-H11都表达显著上调.与腹泻型IBS相比较,便秘型IBS血循环中1种miRNA表达下降,26种miRNA表达上调.结论:IBS血循环具有异常的miRNA表达谱,提示血循环miRNA可作为IBS潜在的诊断标志.

AIM： To investigate the differential expression of circulating microRNAs （miRNAs） between diarrhea-predominant irritable bowel syndrome （D-IBS）/constipation-predominant irritable bowel syndrome （C-IBS） and normal controls to profile abnormally expressed circulating miRNAs in IBS patients. METHODS： Patients were diagnosed with D-IBS or C-IBS based on the Rome III criteria. MiRNA microarray was performed to detect mixed se-rum samples of either three patients with D-IBS, three patients with C-IBS, or three normal controis. After miRNA profiling, clustering analysis was conducted.RESULTS： Compared to normal controls, there were two miRNAs down-regulated and two miRNAs up-regulated in the D-IBS group, and four miRNAs down-regulated and 59 miRNAs up-regulated in the C-IBS group. There was only one miRNA that was expressed differentially in D-IBS patients and 60 miRNAs in C-IBS patients. MiR-23b＊ was down-regulated and HCMV-miR- US5-2 and hsv2-miR-Hll up-regulated in both types of IBS. Compared to D-IBS patients, one miRNA was down-regulated and 26 miRNAs up-regulated in C-IBS patients. CONCLUSION： Abnormal expression profile of circulating miRNAs in IBS patients may provide new biomarkers for diagnosis of this disease.