小柴胡汤对消化不良模型大鼠胃动素和胃泌素的影响

Treatment with Xiaochaihu decoction increases serum levels of motilin and gastrin in rats with dyspepsia

目的:观察小柴胡汤对消化不良模型大鼠(肝郁脾虚型)胃肠激素中胃动素(motilin,MTL)和胃泌素(gastrin,GAS)的影响,分析其治疗消化不良的作用机制.方法:将48只大鼠随机分为4组,每组12只,分别为:空白组、模型组、小柴胡汤组和多潘立酮组.除空白组外其余3组均采用复合病因造模法(慢性束缚应激+过度疲劳+饮食失节)造模,连续21d后造模结束.再按被试因素施加方法每天进行生理盐水、小柴胡汤、多潘立酮灌胃14d.实验第35天给药结束后,禁食不禁水24h,于实验第36天,取血清进行检测.结果:(1)与空白组相比,模型组实验大鼠的血清MTL含量明显降低,差异有高度统计学意义(P=0.00<0.01);与模型组相比,小柴胡汤组(P=0.00)与多潘立酮组(P=0.01)实验大鼠的血清MTL含量明显升高,差异有高度统计学意义(P≤0.01).(2)与空白组相比,模型组实验大鼠的血清GAS含量明显降低,差异有高度统计学意义(P=0.00<0.01);与模型组相比,小柴胡汤组(P=0.01)与多潘立酮组(P=0.00)实验大鼠的血清GAS含量明显升高,差异有高度统计学意义(P≤0.01).结论:小柴胡汤有升高消化不良模型大鼠(肝郁脾虚型)血清MTL、GAS含量的作用.

AIM： To observe the influence of treatment with Xiaochaihu decoction on serum levels of moti lin （MTL） and gastrin （GAS） in a rat model of dyspepsia （syndrome of liver-qi stagnation and spleen-qi deficiency） and to analyze the mechanisms behind therapeutic effects of Xiaochaihu decoction on dyspepsia. METHODS： Forty-eight rats were randomly di- vided into four groups （n = 12 for each）： blank group （BG）, model group （MG）, Xiaochaihu decoction group （XG）, and domperidone group （DG）. Except the BG group, dyspepsia was induced in rats of the other three groups by apply-ing chronic restraint stress±excessive fatigue± irregular food for 21 consecutive days. XG and DG rats were then intragastrically administered with Xiaochaihu decoction and domperidone daily for 14 d, and BG and MG rats were given equal volume of physiological saline. On day 35, the treatment was discontinued and all rats were fasted for 24 h with free access to water. Blood samples were collected on day 36 for testing.RESULTS： Compared to the BG group, serum MTL content in the MG group significantly decreased （P 〈 0.01）. Compared to the MG group, serum MTL content in the XG and DG groups significantly increased （both P≤ 0.01）. Compared to the BG group, serum GAS content in the MG group significantly decreased （P ≤ 0.01）. Compared to the MG group, serum GAS content in the XG and DG groups significantly increased （both P ≤ 0.01）. CONCLUSION： Treatment with Xiaochaihu de coction increased serum levels of MTL and GAS in rats with dyspepsia.