河南汉族人群载脂蛋白E基因型与血脂水平的关系

The relationship between the Apolipoprotein E genotypes and the levels of plasma lipids in Henan Han population

目的 :研究河南汉族人群中载脂蛋白E(ApoE)基因型和等位基因频率以及ApoE表型与血脂水平之间的关系。方法 :运用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)方法检测了 317例健康人的ApoE基因型 ,按标准酶法和免疫学方法测定血脂和载脂蛋白。结果 :研究对象的ε2 ,ε3 和ε4 等位基因频率分别为 0 0 6 8、0 86 9和0 0 6 3,与中国其他地区汉族人群比较差异无显著性。携带ε4 等位基因的个体具有较高的TC及LDL C水平。通过人种间比较发现ε4 等位基因频率在白种人群中较高。结论 :黄种人群ε4 等位基因频率较白种人低 ;

Aim: To study the apolipoprotein E (ApoE) genotypes and allele frequencies and the association between the ApoE genotypes and the serum lipid levels in Chinese population who lived in Henan Methods: The genetic polymorphisms of ApoE in 317 healthy subjects were determined by polymerase chain reaction restriction fragment length polymorohism (PCR RFLP).The serum lipids and apolipoproteins were measured with enzymetic methods and immunologic methods respectively Results: The frequencies of ε 2、ε 3 and ε 4 allele in these subjects were 0 068,0 869 and 0 063 respectively The subjects carrying ε 4 allele were associated with increased concentrations of TC and LDL C Ethnical comparisons had shown that ε 4 allele was more common among Caucasian and less common among Yellow race Conclusion: ApoE ε 4 allele freqeuncy of Yellow race is much less than that of Caucasian;ApoE polymorphism affects the plasma cholesterol level