安泰口服液对荷瘤小鼠白细胞减少症及免疫功能影响的实验研究

Experimental Research on Influence of Antai Oral Liquid on Leucopenia and Immune Function of Tumor-Burdened Mice

目的:探讨安泰口服液(A方组,B方组)对荷瘤小鼠白细胞减少症及免疫功能的影响。方法:(l)建立S180实体瘤模型与骨髓抑制模型。(2)注射环磷酰胺使荷瘤小鼠引起骨髓抑制。(3)取荷瘤小鼠尾静脉注射印度墨汁后的血液,用分光光度计色测光密度值(OD),取肝、脾,称重,计算吞噬指数。(4)荷瘤小鼠腹腔注射绵羊红细胞(SRBC)细胞悬液,测量前后足跖厚度差值(足跖肿胀度)以表示DTH的程度。结果:安泰口服液A方组、B方组与生理盐水组比较外周血白细胞明显提高,差异具有统计学意义(P<0.01)。并且提高荷瘤小鼠单核-巨噬细胞碳廓清的能力。结论:安泰口服液A方组比B方组显著的升高荷瘤小鼠外周血白细胞以及提高免疫功能。以此得到最佳组方的安泰口服液方药,从而为安泰口服液的临床推广运用提供基础研究资料。

Objective： To investigate the oral liquid （ party A, party B group ）of white blood cells in mice to reduce tumor-burdened disease and the influence of the immune function. Methods ： （ 1 ） To establish a S180 solid tumor model and bone marrow inhibition models. （ 2 ）To inject eyelophosphamide to make tumor-burdened mice bone marrow inhibition. （ 3 ）To take blood from tumor-burdened mice tail after intravenous injecting India ink, with spectrophotometer color metering density values （ OD ） and taking liver and spleen, weighing and making index calculation. （ 4 ） Sf/BC was intraperitoneally injected into mice abdomen, thickness measurement （ foot swelling degree ） was tested for detecting DTH. Results ： The party A group, party B group and physiological saline group had obviously improvement in the peripheral white blood cells （ P〈0.O1）. And improvement of mononuelear-maerophages carbon clearance ability of tumor-burdened mice had significance. Conclusion ： Party A group was superior to party B group in the improvement of immune fimetinn and peripheral white blood cells. And thus the best formula of Antai oral liquid has been obtained so as to provide basic research material for promoting the use of oral liquid.