过氧化苯甲酰在诱发金黄地鼠舌癌中的作用

The Function of Benzol Perioxide in the Induction-of Syrian Golden Hamster Tongue Carcinoma by Chemical Carcinogen

目的 :证实在癌变过程中 ,促癌剂和诱癌剂同样有着重要作用。方法 :本实验通过二甲基苯并蒽 (DMBA)涂抹金黄地鼠舌粘膜 ,并用 2 0 %过氧化苯甲酰局部涂抹同一部位 ,每周 2次 ,共 2 0周。同样方法分别涂布DMBA(对照组 )和 2 0 %过氧化苯甲酰 (阴性对照组 )。结果 :对照组成瘤率 90 % (2 7/30 ) ,而实验组为 10 0 % (30 /30 ) ,2组动物均经历了上皮异常增生、原位癌、浸润及转移癌几个阶段 ,但实验组在每一阶段较对照组平均短 2～ 3周左右 ,且有3只金黄地鼠出现颈淋巴结转移。阴性对照组无肿瘤形成。实验组和对照组在大体标本和组织学上无差异 ,镜下呈高分化鳞状上皮细胞癌表现。结论 :过氧化苯甲酰能促进DMBA诱发金黄地鼠舌癌形成。对流行病学调查、癌变发生和预防应同时考虑诱癌剂和促癌剂的作用。

Objective:To testify the function of promotor(benzol perioxide) in the induced tongue carcinogensis of Syrian golden hamster as well as the function of carcinogen (DMBA).Methods: In this study, group 1: 20% benzoyl peroxide in acetone solution was brushed to the right tongue border of Syrian golden hamster, followed by 0 5% 7,12 dimethybenzanthrancene(DMBA) in aceton, twice a week, lasting 20 weeks. Group 2:0 5% 7,12 dimethybenzanthrancene(DMBA) in aceton was painted on the right tongue border of golden Syrian hamster in the manner of group 1. Group 3: 20%benzoyl peroxide in acetone solution was used with mentioned methods.Results: Group 2: showed 90%(27/30) carcinogenesis rate in tongue of Syrian golden hamster. While it was 100%(30/30) of induced tongue carcinoma and 3/30 lymph node metastasis was found in group 1.No tongue carcinogensis was found in group 3. The former two groups had the manifestations of hyperplasia of epithelium cell, carcinoma in situ, invasive and cervical lymph node metastatic carcinoma in the process of experiment, but it was 2 weeks shorter in group 1 than that of group 2. Both groups had the same features under eye and light microscope. The samples were high differentiated squamous cell carcinoma histologically through HE stain. Conclusion: This study indicated that benzoyl peroxide could increase carcinogenesis of golden hamster tongue which induced by DMBA. Consideration of promoting agent should be taken in carcinogenesis as well as carcinogen.\;