黄瓜果皮光泽性状的遗传分析及基因定位研究

Genetic Analysis and Gene Mapping of Glossy Fruit Skin in Cucumber

以黄瓜(Cucumis sativusL.)果皮有光泽自交系‘1101’(P1)为母本,以3个无光泽自交系‘1116’(P2)、‘9930’(P3)、‘1107’(P4)为父本,分别构建6世代遗传群体,对黄瓜果皮光泽性状进行遗传规律分析和基因定位研究。结果表明,黄瓜果皮有光泽性状由显性单基因G控制,有光泽对无光泽为显性。利用‘1101’ב1116’的F2群体,结合分离群体分组分析法(bulked segregate analysis,BSA)筛选得到了30对与黄瓜果皮有光泽基因G相关的SSR标记,将该基因定位到黄瓜第5染色体上,侧翼标记为CS28和SSR15818,遗传距离分别为2.0cM和6.4cM。两侧翼标记之间的物理距离为454kb,在该区域中共预测了177个候选基因。

Glossy fruit skin is one of the highly valuable appearance quality traits related to market values of cucumber （Cucumis sativus L.） . Inbred line ＇ 1101＇ （glossy fruit skin） and inbred line ＇ 1116＇ （dull fruit skin）, ＇9930＇ （dull fruit skin）, ＇1107＇ （dull fruit skin） were used as experiment materials for genetic analysis and gene mapping in this study. Genetic analysis showed that a single dominant nuclear gene, G （glossy fruit skin）, determines the glossy fruit skin trait in cucumber. Bulked segregate analysis （ B SA ） and simple sequence repeat （ S SR） technologies were employed to map G gene of cucumber in ＇1101＇ ~ ＇1116＇ FEpopulation. G gene was mapped to a linkage group with 30 SSR markers, corresponding to chromosome 5 of cucumber. The flanking markers CS28 and SSR15818 were linked to the G gene with genetic distances of 2.0 and 6.4 cM, respectively. The physical distance between the two markers was 454 kb based on the whole genome sequence of cucumber, and there are 177 candidate genes in this region.