前伸下颌后大鼠血中胰岛素含量昼夜节律变化的研究

Circadian Rhythm Changes of the Levels of Endogenous Insulin in Growing Rat's Blood after Functional Mandibular Protrution

目的 :探讨功能矫形治疗对机体全身的影响及髁突局部生长改建与全身生长代谢的关系。方法 :建立功能矫治器前伸大鼠下颌的实验动物模型 ,采用放射免疫学计测手段 ,检测分析前伸大鼠下颌后血中激素类生长因子胰岛素 (Ins)浓度的昼夜节律变化。结果 :自然状态下 ,大鼠血中内源性Ins含量存在昼夜节律性 ,其峰值相位在13:0 2左右 ;戴用功能矫治器后 ,此节律依然存在 ;大鼠血中内源性Ins含量的昼夜节律的峰、谷值相位与髁突软骨组织内源性Ins含量的昼夜节律变化相同。结论 :功能矫治器的使用 ,不会影响机体原有生长代谢的内在节律性 ,且髁突局部的生长改建节律与机体全身的生长代谢节律一致。

Objective: In order to determine whether the metabolism of the body can be influenced by local functional orthopedic treatments and the relationship between the proliferation and translation of condyle and the metabolism of the body, the author investigates the circadian rhythm of the levels of endogenous insulin (Ins) in growing rats' blood and the changes after functional mandibular protrusion in daytime (8:00～20:00) or nighttime (20:00～8:00). Methods: 72 4-week-old SD rats were divided equally and randomly into experimental and control groups, and the rats in experimental groups were put on the functional appliances in daytime (8:00～20:00) or nighttime (20:00～8:00). After 7 days, all rats were sacrificed at 0:00, 4:00, 8:00, 12:00, 16:00, 20:00 in a day. Rodioimmunoassay methods wore used to detect the levels of endogenous insulin in growing rats' blood in every four hours of a day. Results: ①The growing rats' blood displayed a significant circadian rhythm in the levels of endogenous insulin, the acrophase was about 13:02. ②The circadian rhythm still existed after used functional appliance. No significant changes (both mesors and amplitudes) had been observed in the experimental groups comparing with the control groups. ③The circadian rhythm of insulin in blood concerned with that of endogenous insulin in condyle. Conclusion: ①The metabolism of the body can not be influenced by local functional orthopedic treatments. ②The proliferation and translation of condyle concern with the metabolism of the body.